pharm analyt has performed extensive research in various areas of bioanalytics and analytical challenges:
Enantioselective separations of drugs and metabolites in biological matrices
As racemic drugs often have different pharmacodynamic or pharmacokinetic behaviours, separation of enantiomers is either necessary or useful for providing greater insight following administration to animals or humans. In some circumstances, different degrees of protein binding of the two enantiomers can be observed. Since 1988, we have amassed very considerable experience in this field.
Protein binding and its effects
Protein binding of drugs and metabolites gives a better understanding of distribution and elimination, as well as of side effects in some cases. Our experience in this field goes back to the 1970s and 1980s, and has become an increasingly important part of our work since 2003. We use dialysis as the preferred method for testing not only ‘in vitro’ but also ‘in vivo’ samples from animals and humans. The preferred volume is 1 ml, but volumes as small as 0.2 ml are also possible.
Ultra-trace analysis of asthma drugs in human plasma/serum
We have developed determination methods for common asthma drugs such as Formoterol, Budesonide, Albuterol, Fluticasone Propionate, Salmeterol or Ciclesonide, down to 2 - 5 pg/ml plasma. For Formoterol, we have the almost unbeatable determination limit of 400 fg/ml plasma.
We have been working in the field of phytopharmaca for almost 20 years. Structure determination of active substances in extracts by means of HPLC-DAD, HPLC electrochemistry, or HPLC-MS/MS detection following oral, parenteral or dermal administration of extracts of natural substances (e.g. ginkgo biloba, hypericin, menthol, carvone, silymarin, sitosterols,…) to animals and humans.