Poloxamer 188 is a block copolymer of ethylene oxide and propylene oxide.
It is a surfactant and is increasingly used as a replacement for polysorbates. Therefore, it has to be detected more and more often in BDS and FDP samples in the GMP context.
Our team has extensive experience analysing polymers using HPLC-MS/MS with low detection limits, with poloxamer 188 an LLOQ of 1 µg/mL can be reached.
Below are example chromatograms of the blank and a calibration standard with a concentration of 1µg/mL.